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APPENDIX
FORMULAE OF GROWTH AND T E S T MEDIA
A. Growth media
1. Medium B : mod$ed Hayflick medium
(a) Liquid medium
Heart Infusion Broth (Difco)
(Sterilise by autoclaving)
Horse serum (unheated)
Yeast extract (2.5%) (Taylor-Robinson et al., 1963)
Thallium acetate (1 % (w/v) solution)
Penicillin (20 000 IU ml-1)
Deoxyribonucleic acidt (0.2% (w/v) solution)
Adjust p H to 7.8
90.0 ml
20.0 ml
10.0 ml
1.0 ml
0.25 ml
1.2 ml
(b) Solid medium
This is prepared by replacing heart infusion broth with
heart infusion agar (Difco)
2. Medium N
(a) Liquid medium
Bacto brain heart infusion (Difco)
3.7 g
Yeast extract (Difco)
0.5 g
Distilled water
100.0 ml
Sterilise by autoclaving
Horse serum (unheated)
20.0 ml
Yeast extract (25%) (Taylor-Robinson et al., 1963)
10.0 ml
Thallium acetate (1 yo (w/v) solution)
1.0 ml
Penicillin (20 000 I U ml-1)
0.25 ml
Deoxyribonucleic acid (Sigma, 0.2% (w/v) solution)
1.3 ml
Glucose (50% (w/v) solution)
2.0 ml
Adjust pH to 7.8 .
(b) Solid medium
This is prepared by adding 1.4 g Ionagar No. 2 (Code L 12,
t From calf thymus, Sigma Chemical Company, St Louis, Miss., U.S.A.; Type
Oxoid) before autoclaving.
1, Catalogue No. 1501.