C H A P T E R I X
Identification of Mycoplasmas
E. A. FREUNDT, H. ERN0
FA01 WHO Collaborating Centre for Animal Mycoplasmas, Institute of
Medical Microbiology, University of Aarhus, Denmark
AND RUTH M. LEMCKE~
The Lister Institute of Preventive Medicine, University of London, England
I. Introduction .
. 378
11. Taxonomy of the Mycoplasmas .
. 378
A. Class and order
.
. 378
B. Families and genera
.
. 380
C. Species .
. 381
D. Subspecies
.
. 390
E. Present status of classification into species and subspecies
. 390
111. Identification .
. 391
A. Biochemical screening
.
. 395
B. Serological testing .
. 396
IV. Biochemical Methods in Identification
.
. 397
A. Sensitivity to digitonin .
. 397
B. Urease activity
.
. 397
D. Arginine hydrolysis
.
. 399
C. Glucose breakdown
. 398
E. Phosphatase activity
.
. 400
F. Liquefaction of coagulated serum
.
. 401
G. Hydrolysis of aesculin and arbutin .
. 402
A. Comments on antisera
.
. 402
B. Growth inhibition .
. 403
C. Metabolism inhibition
.
. 406
D. Immunofluorescence
.
. 409
E. Direct agglutination
.
. 412
F. Complement fixation
.
. 413
G. Double immunodiffusion
. 420
References
.
. 425
Appendix
.
. 430
A. Growth media
.
. 430
B. Media for biochemical tests
.
. 433
C. Media for metabolism inhibition tests
.
. 434
-t Present address : Agricultural Research Council, Institute for Research on
V. Serological Methods in Identification .
. 402
Animal Disemes, Compton, Newbury, Berks, England.