VI. IDENTIFICATION OF ACTINOAZYCES AND ARACHNIA
3 09
T A B L E X
Serotype specific FZTC conjugates for Actinomyces and Arachnia
Antiserum
Absorbed with
A. bovis, 1
A. bovis, 2
A. odontolyticus, 1
A. odontolyticus, 2
A. israelii, 1
A. israelii, 2
A. naeslundii, 1
A. naeslundii, 2
A. naeslundii, 3
Actinomyces sp.
serotype 963
A. viscosus, 1
A. viscosus, 2
Ar. propionica, 1
Ar. propionica, 2
WVU 116t
WVU 292
WVU 867
WVU 482
WVU 46
WVU 307
wvu 45
WVU 1523
WVU 820
WVU 963
wvu 745
WVU 371
WVU 471
WVU 346
A. bovis, WVU 292
A. bovis, WVU 116
A. odontolyticus, WVU 482
A. odontolyticus, WVU 867
A. israelii, WVU 307; Actinomyces sp.,
WVU 963 (P. avidum)
A. israelii, WVU 46
A. naeslundii, WVU 1523 and WVU 820
A. naeslundii, WVU 45 and WVU 820
A. naeslundii, WVU 45 and WVU 1523
A. israelii, WVU 46; A. naeslundii,
WVU 1523; A. viscosus, WVU 371
A. viscosus, WVU 371
A. viscosus, WVU 745
Ar. propionica, WVU 346
Ar. propionica, WVU 471
t WVU-West Virginia University Culture Collection numbers.
used in sequence, so that cultures are first speciated and then serotyped.
Serotype specific sera are not pooled. If it is desired to prepare a conjugate
which is both species and serotype specific, the absorptions indicated in
both tables should be uscd. This requires multiple absorptions of a single
serum and msy result in loss of titre. We have made such sera using batch
absorption methods, but they are less satisfactory for routine culture
identification than sequential use of two sera and much more difficult to
prepare. Column methods should make preparation of these reagents more
practical. Conjugates absorbed to be both species and serotype specific
may be of value in direct staining of clinical material and would be most
useful in ecological surveys.
D. Reactions with morphologically similar bacteria
Actinomyces show very few cross-reactions with other genera and those
which do occur are generally strain variable and low titred (Lambert et al.,
1967; Holmberg and Forsum, 1973; Slack and Gerencser, 1975). Only
minor cross-reactions have been reported between Actinomyces and
Aruchniu. Holmberg and Forsum (1973) observed 1 + fluorescence of
Aruchniu propimica antigen with low dilutions of A. israelii serotype 1
3 09
T A B L E X
Serotype specific FZTC conjugates for Actinomyces and Arachnia
Antiserum
Absorbed with
A. bovis, 1
A. bovis, 2
A. odontolyticus, 1
A. odontolyticus, 2
A. israelii, 1
A. israelii, 2
A. naeslundii, 1
A. naeslundii, 2
A. naeslundii, 3
Actinomyces sp.
serotype 963
A. viscosus, 1
A. viscosus, 2
Ar. propionica, 1
Ar. propionica, 2
WVU 116t
WVU 292
WVU 867
WVU 482
WVU 46
WVU 307
wvu 45
WVU 1523
WVU 820
WVU 963
wvu 745
WVU 371
WVU 471
WVU 346
A. bovis, WVU 292
A. bovis, WVU 116
A. odontolyticus, WVU 482
A. odontolyticus, WVU 867
A. israelii, WVU 307; Actinomyces sp.,
WVU 963 (P. avidum)
A. israelii, WVU 46
A. naeslundii, WVU 1523 and WVU 820
A. naeslundii, WVU 45 and WVU 820
A. naeslundii, WVU 45 and WVU 1523
A. israelii, WVU 46; A. naeslundii,
WVU 1523; A. viscosus, WVU 371
A. viscosus, WVU 371
A. viscosus, WVU 745
Ar. propionica, WVU 346
Ar. propionica, WVU 471
t WVU-West Virginia University Culture Collection numbers.
used in sequence, so that cultures are first speciated and then serotyped.
Serotype specific sera are not pooled. If it is desired to prepare a conjugate
which is both species and serotype specific, the absorptions indicated in
both tables should be uscd. This requires multiple absorptions of a single
serum and msy result in loss of titre. We have made such sera using batch
absorption methods, but they are less satisfactory for routine culture
identification than sequential use of two sera and much more difficult to
prepare. Column methods should make preparation of these reagents more
practical. Conjugates absorbed to be both species and serotype specific
may be of value in direct staining of clinical material and would be most
useful in ecological surveys.
D. Reactions with morphologically similar bacteria
Actinomyces show very few cross-reactions with other genera and those
which do occur are generally strain variable and low titred (Lambert et al.,
1967; Holmberg and Forsum, 1973; Slack and Gerencser, 1975). Only
minor cross-reactions have been reported between Actinomyces and
Aruchniu. Holmberg and Forsum (1973) observed 1 + fluorescence of
Aruchniu propimica antigen with low dilutions of A. israelii serotype 1