C H A P T E R V
Bacteriophage Typing of Shigella
TOM BERGAN
Department of Microbiology, Institute of Pharmacy, and Department of
Microbiology, Aker Hospital, University of Oslo, Oslo, Norway
I. General Introduction
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. 178
11. Taxonomy of Shigella
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. 179
111. Serology of Shigella
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. 180
A. Major serogroups of Shigella .
. 180
B. Antigenic variability
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. 185
C. 0-antigen cross-reactions between Shigella and other species 185
D. Thermolabile antigens .
. 186
E. Serology and biotype .
. 187
IV. Bacterium-Phage Interactions .
. 188
A. Source of Shigella bacteriophages .
. 188
B. Modification and restriction of phage DNA
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. 188
C. Lysogenic conversion of bacterial antigens
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. 189
D. Phage receptor chemistry
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. 190
V. Properties of Shigella Phages .
. 190
A. Morphology of phages .
. 190
B. Serology of phages
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. 190
C. Phage sensitivity to physical agents .
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. 191
VI. Methods of Phage Typing
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. 191
A. Development of lysotyping .
. 191
B. Phage typing of S. dysenteriae
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. 191
C. Phage typing of S. flexneri .
. 192
D. Phage typing of S. boydii
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. 214
E. Phage typing of S. sonnei
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. 214
VII. Reproducibility and Stability of Type Patterns .
. 277
A. Molecular basis of instability .
. 277
B. Type stability in vitro .
. 277
C. Type stability in vivo .
. 277
D. Type method of reproducibility
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. 278
VIII. Epidemiological Use
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. 278
IX. Opinion on Place of Shigella Phage Typing
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. 280
X. Future Trends
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. 281
References
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. 282
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