168
A. SARAGEA ET AL.
(g) Pisu’s modified medium.
(h) Hiss’s base.
(i) Blake-Christensen’s liquid medium or slants.
(j) Loeffler slants.
(k) Brain-heart agar base (commercial Difco or self-prepared).
4. Biological materials and solutions
(a) Normal bovine or horse serum.
(b) Horse diphtherial antitoxin either plain or purified (500-1000
(c) Sheep or rabbit erythrocytes.
(d) L-Cystine, 1% solution.
(e) Sodium citrate, 0.7 M solution.
(f) Calcium chloride, molar, solution.
(g) Potassium tellurite, 1% solution (for CTBA and Tinsdale).
(h) Potassium tellurite 2% solution (for ECST).
(i) Defibrinated sheep erythrocytes formolised (for each 100 ml erythro(j) NaOH ~ / 1 0
(for Tinsdale).
(k) L-Cystine 0.4 ~ / 1 0
HCl (for Tinsdale).
(1) Sodium thiosulphate solution, 2.5 g% (for Tinsdale).
(m) Carbohydrate solutions : glucose, sucrose, mannite, maltose, levulose, dextrin and glycogen 10% solutions; starch, 4% solution.
(n) Lead acetate, 10% solution (for Pisu’s medium).
(0) Phenol red sen, 1/500 solution (for Blake-Christensen medium).
(p) Thiamine solution, 0.01%.
(r) Tryptophan solution, 0.5y0.
(s) Alkaline Methylene blue solution (Loeffler’s stain).
(t) Gram stains.
AU/ml).
cytes, 0.125 ml formalin) (for Tinsdale).
5. Animals
Guinea pigs (250 g weight).
Rabbits (2500-3000 g weight) with white skin.
6. General remarks
(a) It is strongly recommended that each new batch of medium (even if
commercial media) should be tested before being introduced into use with
reference strains. Thus:
(b) Reference strains : biotypes gravis, mitis, intermedius, toxigenic and
non-toxigenic, C. ulcerans, C. hofmannii, C. xerosis must always be available,
in all the laboratories.
A. SARAGEA ET AL.
(g) Pisu’s modified medium.
(h) Hiss’s base.
(i) Blake-Christensen’s liquid medium or slants.
(j) Loeffler slants.
(k) Brain-heart agar base (commercial Difco or self-prepared).
4. Biological materials and solutions
(a) Normal bovine or horse serum.
(b) Horse diphtherial antitoxin either plain or purified (500-1000
(c) Sheep or rabbit erythrocytes.
(d) L-Cystine, 1% solution.
(e) Sodium citrate, 0.7 M solution.
(f) Calcium chloride, molar, solution.
(g) Potassium tellurite, 1% solution (for CTBA and Tinsdale).
(h) Potassium tellurite 2% solution (for ECST).
(i) Defibrinated sheep erythrocytes formolised (for each 100 ml erythro(j) NaOH ~ / 1 0
(for Tinsdale).
(k) L-Cystine 0.4 ~ / 1 0
HCl (for Tinsdale).
(1) Sodium thiosulphate solution, 2.5 g% (for Tinsdale).
(m) Carbohydrate solutions : glucose, sucrose, mannite, maltose, levulose, dextrin and glycogen 10% solutions; starch, 4% solution.
(n) Lead acetate, 10% solution (for Pisu’s medium).
(0) Phenol red sen, 1/500 solution (for Blake-Christensen medium).
(p) Thiamine solution, 0.01%.
(r) Tryptophan solution, 0.5y0.
(s) Alkaline Methylene blue solution (Loeffler’s stain).
(t) Gram stains.
AU/ml).
cytes, 0.125 ml formalin) (for Tinsdale).
5. Animals
Guinea pigs (250 g weight).
Rabbits (2500-3000 g weight) with white skin.
6. General remarks
(a) It is strongly recommended that each new batch of medium (even if
commercial media) should be tested before being introduced into use with
reference strains. Thus:
(b) Reference strains : biotypes gravis, mitis, intermedius, toxigenic and
non-toxigenic, C. ulcerans, C. hofmannii, C. xerosis must always be available,
in all the laboratories.