C I l A P T E H I
Bacteriocin, Bacteriophage and other
Epidemiological Typing Methods
for the Genus Clostridium
D. E. MAHONY
Halifax, N o v a Scotia, Canada
Department of Microbiology, Faculty of Medicine, Dalhousie University,
I. Introduction .
11. Approaches to Bacteriocin Typing .
111. Growth and Identification of Clostridia
.
IV. Nature and Mode of Action of Clostridial Bacteriocins
.
A. Bacteriocins of C. perfringens .
B. Bacteriocins of C. botulinum .
C . Other bacteriocins.
.
V. Bacteriophages of Clostridium .
A. Bacteriophages of C. perfringens and other clostridia associated with gas gangrene .
B. Bacteriophages of other clostridia .
A. Preparation of bacteriocin
.
B. Titration of bacteriocin .
VII. Technique of Bacteriocin Typing
.
VI. Production of Bacteriocin for Typing of C. perfringens.
.
VIII. Interpretation of Bacteriocin Typing.
.
A. Reading of plates .
B. Patterns of susceptibility
.
C. Variability .
IX. Potential Use of Bacteriocin Typing of C. perfringens .
X.
XI.
Other Epidemiological Methods of Typing Clostridia: Serology,
Toxins
Bacteriophage and Bacteriocin Typing of Clostridium : Summary
and Overview
.
References
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I. INTRODUCTION
Many of the bacteria classified as clostridia are harmless. Their habitat
is in the soil and intestinal tract of man and animals; yet some of these