2.11.
BOTANICAL
MATERIAL
33
2.11. Botanical Material
The fact that plant material is likely to be difficult to preserve has led
people to experiment with variations of fixatives, some of which are discussed in the next chapter. McLean (1960), however, emphasizes that the
presence of large turgid vacuoles creates a problem not found in animal
cells. He supposes that the destruction of the differential permeability of
the tonoplast as the fixative penetrates may result in the sudden release of
acidic vacuolar fluid into the as yet incompletely fixed cytoplasm. He
proposes that the turgor pressure be released before fixation by treating
the plant tissue with suitable glucose solutions until plasmolysis is incipient. This clearly has proved effective in some instances.
LITERATURE CITED
Ito, S. (1962a). In "The Interpretation of Ultrastructure" (R. J. C. Harris, ed.), p. 129.
Academic Press, New York.
Ito, S. (1962b). "Electron Microscopy," Proc. 5th Intern. Congr. Electron Microscopy,
Vol. 2, p. L-5. Academic Press, New York.
Low, F. N., and Freeman, J. A. (1958). "Electron Microscopic Atlas of Normal and
Leukemic Human Blood." McGraw-Hill, New York.
McLean, J. D. (1960). 4th Intern.
Conf. Electron
Microscopy,
Berlin,
1958, 2, 27.
Springer, Berlin.
Palade, G. E. (1952). J. Exptl.
Med.
95, 171.
Palade, G. E. and Siekovitz, P. (1956). /. Biophys.
Biochem.
Cytol. 2, 171.
Palay, S. L., McGee-Russell, S. M., Spencer, G., and Grillo, M. A. (1962). /. Cell
Biol.
12, 385.
Pallie, W., and Pease, D. C. (1958). /. Ultrastructure
Research
2, 1.
Sjostrand, F. S. (1953). /. Cellular
Comp. Physiol.
42, 15 and 45.
BOTANICAL
MATERIAL
33
2.11. Botanical Material
The fact that plant material is likely to be difficult to preserve has led
people to experiment with variations of fixatives, some of which are discussed in the next chapter. McLean (1960), however, emphasizes that the
presence of large turgid vacuoles creates a problem not found in animal
cells. He supposes that the destruction of the differential permeability of
the tonoplast as the fixative penetrates may result in the sudden release of
acidic vacuolar fluid into the as yet incompletely fixed cytoplasm. He
proposes that the turgor pressure be released before fixation by treating
the plant tissue with suitable glucose solutions until plasmolysis is incipient. This clearly has proved effective in some instances.
LITERATURE CITED
Ito, S. (1962a). In "The Interpretation of Ultrastructure" (R. J. C. Harris, ed.), p. 129.
Academic Press, New York.
Ito, S. (1962b). "Electron Microscopy," Proc. 5th Intern. Congr. Electron Microscopy,
Vol. 2, p. L-5. Academic Press, New York.
Low, F. N., and Freeman, J. A. (1958). "Electron Microscopic Atlas of Normal and
Leukemic Human Blood." McGraw-Hill, New York.
McLean, J. D. (1960). 4th Intern.
Conf. Electron
Microscopy,
Berlin,
1958, 2, 27.
Springer, Berlin.
Palade, G. E. (1952). J. Exptl.
Med.
95, 171.
Palade, G. E. and Siekovitz, P. (1956). /. Biophys.
Biochem.
Cytol. 2, 171.
Palay, S. L., McGee-Russell, S. M., Spencer, G., and Grillo, M. A. (1962). /. Cell
Biol.
12, 385.
Pallie, W., and Pease, D. C. (1958). /. Ultrastructure
Research
2, 1.
Sjostrand, F. S. (1953). /. Cellular
Comp. Physiol.
42, 15 and 45.