Subject Index
A
Aberration
chromatic, 281
spherical, 281
Accelerator 964C,
108
Acetaldehyde, 57
Acetone
dehydration, 85, 118
effect on staining, 218
flotation
fluid,
163, 165, 174-175, 182183, 186
transitional solvent, 87, 118
Acid dyes, 258-259
Acrolein, 50, 57, 59
Acrylic aldehyde (see acrolein)
Adhesive for grids, 208
Aerosols, 329-331, 349, 352
Afzelius' osmium tetroxide in carbon tetrachloride, 44-45
Albumen, wetting agent (see proteins)
Alcohol
dehydration, 84-85, 86, 354
stain vehicle, 218, 225, 235, 237
Aldehyde fuchsin, 259
Aldehydes (see also particular aldehydes)
duration of fixation, 20, 354
fixation, 14, 15, 20, 22, 23-29, 35, 50-63,
67, 354-356
histochemical
applications, 35,
50-51,
122, 123, 124, 126, 245-247
penetration, 20, 29, 56
staining, 225, 243-245
Alignment (also see specific part)
current, 282-283, 284
voltage, 281-284
Alkaline lead hydroxide stain, 82, 211,
217-234, 243-245 (Figs. 4, 5, 10, 11,
25, 27, 39, 40)
alcoholic solution, 218, 225
autoradiographic stain, 255, 256
contamination, 221, 225, 227-229
dependence on fixative, 243-245
following uranyl stains, 231-234
mechanics of stain, 219, 230, 243-245
reaction with osmium tetroxide, 243
secondary staining, 231-234
Allergic toxicity, 91-92
Aluminized
fluorescent
screen, 308
Ammonium acetate, volatile salt, 327
Ammonium
benzoate, volatile salt, 327328
Anode
alignment, 282, 284, 288-289
cleaning, 289
contamination, 289, 296-297
Antibody stains, 247-250
Apertures (see particular apertures, objective, projector, or condenser)
Aquon, 122, 123
Araldite
embedding, 83, 105, 108-110, 121, 355
sectioning, 110, 123, 178 (Figs. 3, 4, 5, 29,
39)
solvents, 262-263
staining, 217, 224, 355
thick section staining, 259-260
Araldite M, 108
Araldite 502, 108. 110
Araldite 6005, 110
Astigmatism (see objective lens)
Autoradiography, 4, 250-256
alkaline lead stain, 255-256
carbon-14, 251
development, 254-255
embedments, 252
emulsions, 252
exposure, 251-252, 254
frozen-substituted tissue, 75
resolution, 250-251, 256
statistical analysis, 256
sulphur-35, 251
tritium, 251
Azodiisobutyronitrile, catalyst, 104, 126
Azure, 260
B
Backing films (see also replicas)
336, 342, 343, 344
367
A
Aberration
chromatic, 281
spherical, 281
Accelerator 964C,
108
Acetaldehyde, 57
Acetone
dehydration, 85, 118
effect on staining, 218
flotation
fluid,
163, 165, 174-175, 182183, 186
transitional solvent, 87, 118
Acid dyes, 258-259
Acrolein, 50, 57, 59
Acrylic aldehyde (see acrolein)
Adhesive for grids, 208
Aerosols, 329-331, 349, 352
Afzelius' osmium tetroxide in carbon tetrachloride, 44-45
Albumen, wetting agent (see proteins)
Alcohol
dehydration, 84-85, 86, 354
stain vehicle, 218, 225, 235, 237
Aldehyde fuchsin, 259
Aldehydes (see also particular aldehydes)
duration of fixation, 20, 354
fixation, 14, 15, 20, 22, 23-29, 35, 50-63,
67, 354-356
histochemical
applications, 35,
50-51,
122, 123, 124, 126, 245-247
penetration, 20, 29, 56
staining, 225, 243-245
Alignment (also see specific part)
current, 282-283, 284
voltage, 281-284
Alkaline lead hydroxide stain, 82, 211,
217-234, 243-245 (Figs. 4, 5, 10, 11,
25, 27, 39, 40)
alcoholic solution, 218, 225
autoradiographic stain, 255, 256
contamination, 221, 225, 227-229
dependence on fixative, 243-245
following uranyl stains, 231-234
mechanics of stain, 219, 230, 243-245
reaction with osmium tetroxide, 243
secondary staining, 231-234
Allergic toxicity, 91-92
Aluminized
fluorescent
screen, 308
Ammonium acetate, volatile salt, 327
Ammonium
benzoate, volatile salt, 327328
Anode
alignment, 282, 284, 288-289
cleaning, 289
contamination, 289, 296-297
Antibody stains, 247-250
Apertures (see particular apertures, objective, projector, or condenser)
Aquon, 122, 123
Araldite
embedding, 83, 105, 108-110, 121, 355
sectioning, 110, 123, 178 (Figs. 3, 4, 5, 29,
39)
solvents, 262-263
staining, 217, 224, 355
thick section staining, 259-260
Araldite M, 108
Araldite 502, 108. 110
Araldite 6005, 110
Astigmatism (see objective lens)
Autoradiography, 4, 250-256
alkaline lead stain, 255-256
carbon-14, 251
development, 254-255
embedments, 252
emulsions, 252
exposure, 251-252, 254
frozen-substituted tissue, 75
resolution, 250-251, 256
statistical analysis, 256
sulphur-35, 251
tritium, 251
Azodiisobutyronitrile, catalyst, 104, 126
Azure, 260
B
Backing films (see also replicas)
336, 342, 343, 344
367